引言
胚胎着床是胚胎与子宫内膜建立紧密联系的过程,是成功妊娠的关键步骤。但是在一些极端的条件下,哺乳动物利用胚胎滞育(embryonic diapause),也称延迟着床(Delayed implantation)来延长妊娠时间,进而确保胎儿在有利的环境中出生【1,2】。在小鼠中,胚胎滞育起始于胚胎期(Embryonic day, E)4.5天的囊胚期(Blastocyst),滞育的胚胎具有形态发生停止和生理活动降低等特征【1】。科学家可以在小鼠上人工诱导滞育。具体而言,在受精后的第3天清晨,通过卵巢切除(Ovariectomy)和孕酮(Depo)注射诱导胚胎滞育【3】。经单剂量雌二醇(Estrogen)激活后,滞育的胚胎可以正常植入和发育【4】。然而,由于胚胎的取材限制和数据获取的技术难度,对小鼠胚胎滞育的调控机制尚不清楚。
2024年7月23日,德国马克思普朗克分子生物医学研究所Ivan Bedzhov课题组在Cell Stem Cell上发表了文章Analyzing embryo dormancy at single-cell resolution reveals dynamic transcriptional responses and activation of integrin-Yap/Taz prosurvival signaling。通过单细胞RNA测序技术,首次绘制了小鼠胚胎进入,维持和退出滞育,以及植入前和植入后的转录组图谱,并揭示了Integrin-Yap/Taz信号通路在胚胎滞育中的重要作用。


参考文献
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文章来源|“BioArt”
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