2023年“细胞培养”相关文章
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导言
我们汇编了Bio-protocol期刊在过去一年中积累的“细胞培养”相关的实验方案。这些方案是来自全球杰出科学家的分享成果。细胞培养作为生命科学的基石,对于我们探索细胞行为、疾病机理以及新药研发具有不可替代的重要性。我们提供的实验方案不仅经过了验证,且详细易于操作。根据用户反馈,有91%的用户能够成功地复现这些实验,这充分证明了我们方案的可靠性。
我们计划定期更新这一系列方案,以期为您的研究提供强有力的支持和灵感启发。
● Protocol for 3D Bioprinting Mesenchymal Stem Cell–derived Neural Tissues Using a Fibrin-based Bioink
使用基于纤维蛋白的生物墨水对间充质干细胞衍生的神经组织进行3D生物打印的方案
链接:https://doi.org/10.21769/BioProtoc.4663
作者:Milena Restan Perez、Nadia Z. Masri、Jonathan Walters-Shumka、Sarah Kahale、Stephanie Michelle Willerth
● Three-dimensional Co-culture Model for Live Imaging of Pancreatic Islets, Immune Cells, and Neurons in Agarose Gel
用于琼脂糖凝胶中胰岛、免疫细胞和神经元实时成像的三维共培养模型
链接:https://doi.org/10.21769/BioProtoc.4852
作者:Elke M. Muntjewerff、Vijay S. Josyula、Gustaf Christoffersson
● Mouse Corneal Epithelial and Stromal Cell Isolation and Culture
小鼠角膜上皮和基质细胞的分离和培养
链接:https://doi.org/10.21769/BioProtoc.4829
作者:Yingnan Zhang、Lijun Zhang、Yongqing Liu
● Human Schwann Cells in vitro I. Nerve Tissue Processing, Pre-degeneration, Isolation, and Culturing of Primary Cells
体外人雪旺细胞 I. 神经组织的加工、预变性、分离和原代细胞培养
链接:https://doi.org/10.21769/BioProtoc.4748
作者:Gabriela I. Aparicio、Paula V. Monje
● The Development of an Advanced Model for Multilayer Human Skin Reconstruction In Vivo
开发先进的多层人体活体皮肤重建模型
链接:https://doi.org/10.21769/BioProtoc.4919
作者:Maryna Pavlova、Velmurugan Balaiya、Jocelyn C. Flores、Michael Ferreyros、Katie Bush、Amy Hopkin、Igor Kogut、Dennis R. Roop、Ganna Bilousova
● Preparation of Human Kidney Progenitor Cultures and Their Differentiation into Podocytes
人肾脏祖细胞培养物的制备及其向足细胞的分化
链接:https://doi.org/10.21769/BioProtoc.4757
作者:Maria Elena Melica、Maria Lucia Angelotti、Giulia Antonelli、Anna J. Peired、Carolina Conte、Letizia De Chiara、Benedetta Mazzinghi、Elena Lazzeri、Laura Lasagni、Paola Romagnani
● Isolation of Embryonic Cardiomyocytes and Cell Proliferation Assay Using Genetically Engineered Reporter Mouse Model
使用基因工程报告小鼠模型分离胚胎心肌细胞并进行细胞增殖测定
链接:https://doi.org/10.21769/BioProtoc.4802
作者:Maren Beall、Deqiang Li、Jihyun Jang
● A Large-format Polyacrylamide Gel with Controllable Matrix Mechanics for Mammalian Cell Culture and Conditioned Media Production
用于哺乳动物细胞培养和条件培养基生产的具有可控基质力学的大规格聚丙烯酰胺凝胶
链接:https://doi.org/10.21769/BioProtoc.4807
作者:Catherine S. Millar-Haskell、Jason P. Gleghorn
● Isolation and Culture of Neural Stem/Progenitor Cells from the Hippocampal Dentate Gyrus of Young Adult and Aged Rats
青年和老年大鼠海马齿状回神经干/祖细胞的分离和培养
链接:https://doi.org/10.21769/BioProtoc.4843
作者:Mina Afhami、Morteza Behnam-Rassouli、Ali Gorji、Saeed Karima、Koorosh Shahpasand
● A Methodology for the Enzymatic Isolation of Embryonic Hypothalamus Tissue and Its Acute or Post-Culture Analysis by Multiplex Hybridisation Chain Reaction
胚胎下丘脑组织的酶分离方法及通过多重杂交链式反应进行的急性或培养后分析
链接:https://doi.org/10.21769/BioProtoc.4898
作者:Kavitha Chinnaiya、Marysia Placzek