你清楚AnnexinVFITC,AnnexinVFluorescein的基本工作原理吗

新研博美chemical 2025-03-26 17:24:14

Annexin V-FITC is a recombinant human Annexin V protein that binds to fluorescein isothiocyanate ( FITC ). It is mainly used to detect phosphatidylserine ( PS ) that appears on the surface of the cell membrane during apoptosis.

Keywords : Annexin V FITC, Annexin V Fluorescein, Fluorescence detection, Flow cytometry, Propidium iodide ( PI ), Annexin V, Phosphatidylserine ( PS )

| 基本信息

[英文名称]Annexin V FITC,Annexin V Fluorescein

[中文名称]异硫氰酸荧光素标记的膜联蛋白V

[CAS号]N/A

[分子式]N/A

[分子量]N/A

[外观]液体

[荧光]FITC

[规格]10mg、25mg、50mg

| 工作原理

Annexin V can specifically bind to phosphatidylserine ( PS ) exposed on the outside of the cell membrane, which is an obvious feature of early apoptosis. Annexin V-FITC complex can be formed by labeling Annexin V with FITC. The complex can emit green fluorescence under the excitation of specific wavelength light, so that the occurrence of apoptosis can be detected by fluorescence microscopy or flow cytometry.

| 应用领域

AnnexinV-FITC使用荧光素异硫氰酸酯(FITC)偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。与碘化丙啶(PI)组合可以区分早期凋亡细胞(annexin V阳性, PI阴性),坏死细胞(annexin V阳性, PI阳性),以及活细胞(annexin V阴性, PI阴性)。

AnnexinV-FITC used fluorescein isothiocyanate ( FITC ) -conjugated annexin V to label phosphatidylserine sites on the cell membrane surface. Combined with propidium iodide ( PI ), it can distinguish early apoptotic cells ( annexin V positive, PI negative ), necrotic cells ( annexin V positive, PI positive ), and living cells ( annexin V negative, PI negative ).

| 注意事项

[储存]-20°C避光

[供应商]陕西新研博美生物科技有限公司

[声明]试剂仅供科研,不可用于人体!

| 相关试剂

Click-iT Plus EdU Alexa Fluor 350

AF633 Wheat Germ Agglutinin,AF633 WGA

Oregon Green 488 Wheat Germ Agglutinin

Qdot 655 Wheat Germ Agglutinin

Tetramethylrhodamine Wheat Germ Agglutinin,

Texas Red-X Wheat Germ Agglutinin

Alexa Fluor 350 NeutrAvidin,AF 350 NeutrAvidin

Annexin V Alexa Fluor 488,Annexin V AF488

Annexin V Alexa Fluor 568,Annexin V AF568

(本文内容由陕西新研博美生物科技有限公司的小编xj整理)

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